Figure 2.

PMSCs treated with IGF-2 showed qualitatively increased immunofluorescence for MHC at 14 days. Compared to PMSCs cultured under muscle differentiation alone, PMSCs treated with IGF-1 or IGF-2 showed similar OCT4 immunoreactivity (red-Alexa, λ = 568 nm). However, IGF-1 supplementation increased Pax3/7 IR (green-Alexa, λ = 488 nm), decreased MyoD IR, with no change in MHC IR (red-Alexa, λ = 568 nm). In contrast, IGF-2 treatment did not alter Pax3/7 IR (green-Alexa, λ = 488 nm) or MyoD IR, but increased MHC IR (red-Alexa, λ = 568 nm). Nuclei, stained with Hoechst dye (blue, λ = 340 nm). Immunocytochemistry was performed in triplicate with each antibody.