Figure 3.

PMSCs cultured under skeletal muscle differentiation conditions treated with IGF-1 showed an increased frequency of cells with high ALDH activity, while cells treated with IGF-2 showed a decreased frequency. Compared to PMSCs under muscle differentiation conditions, cells treated with IGF-1 showed increased frequency of cells with high ALDH activity, while cells treated with IGF-2 showed decreased frequency of cells with high ALDH activity. Data is presented as the mean ± SEM of 3 independent experiments. Two-way ANOVA with Bonferroni's multiple comparison test was performed to determine ∗P < 0.05 and ∗∗∗P < 0.001.