Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The primer sequences of mRNAs.
| Gene | Oligonucleotide primers (5′-3′) | Product size (bp) | Annealing (°C) | Cycle | |
|---|---|---|---|---|---|
| TNF-α | F | CGTCAGCCGATTTGCCATTT | 88 | 60 | 40 |
| R | TCCCTCAGGGGTGTCCTTAG | 60 | 40 | ||
|
| |||||
| IFN-γ | F | GAGGAACTGGCAAAAGGACG | 132 | 60 | 40 |
| R | AGGTGCGATTCGATGACACT | 60 | 40 | ||
|
| |||||
| GADPH | F | AAGGTCGGTGTGAACGGATT | 70 | 60 | 40 |
| R | CTTTGTCACAAGAGAAGGCAGC | 60 | 40 | ||
Note. F: forward; R: reverse.
