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. 2017 Dec 28;18:54. doi: 10.1186/s12865-017-0237-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — SDS-PAGE and circular dichroim analysis of CCL8 after digestion. a SDS-PAGE analysis of CCL8. Lane M represents protein standard markers. Lane 1 is His-tagged CCL8 fusion protein; lane 2, digested mixture of His-tagged CCL8 fusion protein; lane 3, purified CCL8 protein after the second Ni affinity chromatography; and lane 4 represents the mixture of undigested fusion protein, fusion part of CCL8 and His₆-TEV enzymes captured on column, respectively. b Mass spectrometry analysis of the purified CCL8. c Circular dichroism analysis of recombinant CCL8