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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Placenta. 2017 Nov 10;61:33–38. doi: 10.1016/j.placenta.2017.11.004

Figure 2. Trophoblastic extracellular vesicles or C19MC miRNAs do not enhance the expression of ISGs.

Figure 2

(A) RT- qPCR analysis of IFI44L expression in U2OS cells exposed to the PHT-derived extracellular vesicles apoptotic bodies (AB), microvesicles (MV), or exosomes (EX). PHT conditioned medium (CM) was used as a positive control. Results are expressed relative to non-conditioned medium (CTRL). (B) RT-qPCR analysis of the expression of the ISGs IFI44L, IFIT1, IFIT2 and OAS1 in U2OS cells transfected with either 40 nM of miR-517a, miR-516b-5p, miR-512-3p, or an equal concentration of control non- targeting miRNA (Neg CTRL). 10 ng IFN lambda-1 was used as a positive control. Experiments were performed three independent times. Data are presented as fold-change relative to control non-targeting miRNA, and shown as mean ±SD (* p<0.05). Differences were determined using ANOVA, with post hoc Bonferroni correction.