Fig. 3.

Overexpression of Survivin in glioma cells affects cell cycle and proliferation in a p53 dependent manner. a Western blot analysis showing cell lysates from U87-MG and U87-MG^shp53 after transduction with Survivin and control vectors. The membranes were blotted with anti-p53 (53 kDa), anti-p53s15 (53 kDa), anti-p21^waf/cip (21 kDa), anti-γH2AX (16 kDa), anti-cyclin D1 (37 kDa) and E (42 kDa singlets +50 kDa doublets) antibodies and the signals comparing control and Survivin cells were measured. The relative band density (fold increase) obtained from the densitometric analysis and normalized to the corresponding control is depicted. α-actin (42 kDa) was used as an internal loading control. b Representative BrdU-incorporation assays of U87-MG (left panel) and U87-MGs^hp53 cells (right panel) with overexpression of Survivin (bottom) compared to the empty vector-transduced controls (top). The overall amount of BrdU-positive fractions as well as the relative levels of BrdU⁺ and BrdU⁻ cells in fractions with DNA contents of 2n, 4n, 8n and >8n are depicted. c Proliferation index of the U87-MG and U87-MG^shp53 cells transduced with Survivin and empty vector controls. Depicted are mean values ± SD. **p < 0.01. d BrdU-incorporation in U87-MG and U87-MG^shp53 cell fractions with DNA content >4n. Depicted are mean values ± SD. **p < 0.01. All data were collected 72 h after transduction of cells