Fig. 2.

GOLM1 knockdown inhibits glioma progression in U251 and A172 cells in vitro. Knockdown efficiency of lentiviral shRNA constructs, sh-GOLM1–1 and sh-GOLM1–2 in U251 and A172 cells assessed with a qRT-PCR and b western blot analysis. U251- and A172-NC and -sh-GOLM1 cell lines evaluated in c CCK8 assay for cell viability. d EdU (red) assays for proliferation rate. Nuclei are stained with DAPI (blue). Scale bar = 100 μm. e Graphic representation of ratios of EdU positive cells in U251- and A172-NC and sh-GOLM1 cells. Data are presented as the mean ± SEM. f Representative images of the morphology of U251- and A172-NC and sh-GOLM1 cells under bright field microscopy. Scale bar = 100 μm. g Images of Transwell assays performed with U251- and A172-NC and sh-GOLM1 expressing cells. Scale bar = 50 μm. h Quantification of invaded and migrated cells in Transwell assays after incubation for 24 h. Data are presented as the mean ± SEM. (**P < 0.01, ***P < 0.001)