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. 2017 Dec 28;18:990. doi: 10.1186/s12864-017-4385-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Diagram of experimental design. Two life stages (adults and larve) were tested in each of the 5 transgenic lines, 4 of them harbouring heterozygous integration of the tTAV constructs (het) and one homozygous (hom). All differential gene expression was assessed between tetracycline ON (tet-ON) and tetracycline OFF (tet-OFF) conditions (double-ended arrows) only within each single strain and life stage to ensure that observed effects are due to different treatments and not different genetic backgrounds