FIGURE 5:

Reduction of ATP usage in cells by migration inhibition. (A) Representative pH-corrected PercevalHR ratios of MDA-MB-231 cells coexpressing PercevalHR and pHRed cultured in a 1.5 mg/ml collagen matrix treated with complete media (CM), vehicle control (DMSO), Y27632 (Y27), LY294002 (LY), ML7, and Latrunculin A (LatA). Quantification of (B) pH-corrected PercevalHR ratiometric signal, (C) 2-NBDG uptake, and (D) ATP hydrolysis for cells cultured in 1.5 mg/ml collagen matrices across inhibitor treatments (n = [B] 40, [C] 45, [D] 30 cells from three independent experiments). Box-and-whisker plots show medians and 25th/75th and 5th/95th percentiles. Bars denote mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 for Wilcoxon rank test for each condition compared with control (CM, complete media). Scale bar = 20 μm.