FIGURE 3:

Knockdown of ELK1, ETS1, and GABPA protects A549 and HeLa cells from Golgi stress–induced cell death. A549 (A, C, E) and HeLa (B, D, F) cells were transduced with lentiviral vectors to stably express shRNAs against ELK1, ETS1, or GABPA and subsequently treated with BFA, GCA, or MON. Avg. Ctrl represents the combined average survival ratio of cells infected with either of two separate control hairpins (shLUC and shRFP). (G) Survival ratios of A549 cells stably expressing shRNAs targeting ELK1 and ETS1. (H) Survival ratios of A549 cells stably expressing shRNAs against ELK1 and GABPA. (G, H) a, significant compared with Avg. Ctrl (Avg. Ctrl = shLUC/shLUC); b, significant compared with the corresponding single knockdown; (A, G, H) BFA = 40 nM, GCA = 1.8 µM, MON = 2 µM; (C, E) BFA = 60 nM, GCA = 1.8 µM, MON = 2 µM; (B, D, F) BFA = 25 nM, GCA = 1.8 µM, MON = 0.5 µM. (A–H) Data are shown as mean and SD of one representative experiment measuring six wells per genotype and condition of three independent experiments; *, a, b, p ≤ 0.05, two-way analysis of variance (ANOVA) with Bonferroni posttest.