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. 2018 Jan 1;29(1):42–52. doi: 10.1091/mbc.E17-06-0418

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© 2018 Baumann et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Overexpression of ELK1 and GABPA/B1 leads to sensitivity to Golgi stress–induced cell death. (A) Survival ratios and expression validation of A549 cells stably expressing FLAG-ELK1, FLAG-GABPA, or FLAG-CREB3, treated with 40 nM BFA. Asterisks (*) denote specific overexpression bands. Avg Ctrl refers to the average survival ratio of control cells infected with either FLAG-RAP2A or FLAG-γTUB. (B) Survival ratios and expression validation of A549 cells stably expressing FLAG-GABPA, FLAG-GABPB1, or the combination of both, treated with 40 nM BFA. (C) Survival ratios of A549 cells stably expressing the combination of FLAG-GABPA/FLAG-GABPB1 or FLAG-GABPA/FLAG-GABPB1/FLAG-ELK1, treated with 40 nM BFA. Expression validation shown for FLAG-GABPA/FLAG-GABPB1/FLAG-ELK1, for all other lines refer to A and B; a, significant compared with Avg Ctrl; b, significant compared with the corresponding single/double overexpression. (A–C) Data are shown as mean and SD of one representative example of three independent experiments measuring six wells per genotype and condition; *, a, b, p ≤ 0.05, two-way ANOVA with Bonferroni posttest. Protein lysates were run on the same gel, and dashed lines in blots indicate where unrelated samples were cropped out.