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. 2017 Nov 10;8(64):107374–107389. doi: 10.18632/oncotarget.22376

Figure 1. PPF-curcumin induces significant chemosensitization towards paclitaxel.

Figure 1

(A) PPF-curcumin is more efficient in augmenting paclitaxel-induced cytotoxicity in HeLa cells, compared to free curcumin. HeLa cells were treated with free curcumin/PPF-curcumin either alone or in combination with paclitaxel for 72 h after pre-treating with curcumin/PPF-curcumin and cell viability assay was performed using MTT. (B) Paclitaxel-induced chromatin condensation is enhanced more efficiently by PPF-curcumin compared to free curcumin. HeLa cells were treated with free curcumin/PPF curcumin either alone or in combination with paclitaxel for 24 h after pre-treating with curcumin/PPF-curcumin. Later, cells were stained with DAPI and images were captured using a fluorescent microscope. The number of condensed nuclei is counted and represented as a graph. (C) PPF-curcumin inhibits clonogenicity of HeLa cells. Cells were treated with different formulations of curcumin (5 μM) either alone or in combination with paclitaxel for 72 h and clonogenic assay was conducted. The lower panel shows the images of individual wells captured at 10X magnification. (D) Paclitaxel-induced caspase cleavage is enhanced by PPF-curcumin more efficiently than free curcumin. HeLa cells were treated with free curcumin or PPF-curcumin either alone or in combination with paclitaxel for 24h after pre-treating with curcumin/PPF-curcumin, subjected to Western blotting followed by detection by ECL.