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. 2017 Nov 10;8(64):107374–107389. doi: 10.18632/oncotarget.22376

Figure 2. Paclitaxel-induced activation of survival signals is down-regulated more efficiently by PPF-curcumin compared to free curcumin.

Figure 2

(A) Paclitaxel-induced phosphorylation of Akt is more efficiently down-regulated by PPF-curcumin than free curcumin. HeLa cells were treated with free curcumin or PPF curcumin either alone or in combination with paclitaxel for 1 h after pre-treating with curcumin/PPF-curcumin and Western blotting and ECL were performed. (B) PPF-curcumin induced significant inhibition of NF-κB activation. HeLa cells were treated with free curcumin or PPF-curcumin for 2 h and/or paclitaxel for 30 min after pre-treating with curcumin/PPF-curcumin, after which nuclear extracts were prepared and EMSA was performed. (C) Paclitaxel-induced up-regulation of Cox-2, Cyclin-D1 and Bcl-2 are down-regulated more efficiently by PPF-curcumin than free curcumin. HeLa cells were treated with free curcumin or PPF-curcumin either alone or in combination with paclitaxel for 24 h, after pre-treating with curcumin/PPF-curcumin and Western blotting and ECL were performed. (D) Paclitaxel-induced up-regulation of IAPs is more proficiently down-regulated by PPF-curcumin than free curcumin. HeLa cells were treated with free curcumin or PPF-curcumin either alone or in combination with paclitaxel for 24 h, after pre-treating with curcumin/PPF-curcumin and Western blotting and ECL were performed. (E) Paclitaxel-induced phosphorylation of Erk 1/2, p38 and JNK are more effectively down-regulated by PPF-curcumin compared to free curcumin. HeLa cells were treated with free curcumin or PPF-curcumin and/or paclitaxel for 15 min, after pre-treating with curcumin/PPF-curcumin and subjected to Western blotting followed by detection by ECL. All the blots were quantified and band density of individual bands are indicated in the blots. (F) PPF-curcumin induced significant inhibition of AP-1 activation. HeLa cells were treated with free curcumin or PPF-curcumin for 2 h and/or paclitaxel for 30 min after pre-treating with curcumin, after which nuclear extracts were prepared and EMSA was performed.