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. 2017 Nov 17;8(64):107423–107440. doi: 10.18632/oncotarget.22475

Figure 5. Proliferation and cell size in 2D and 3D cultures.

Figure 5

(A) 3000 cells were seeded to generate the 2D and 3D cultures. Every second day the cells were harvested by trypsinization for determining cell number and viability. Each data point represents the mean ± s.d. of three independent experiments. (B) Cell size distributions were assessed by measuring the diameter of >100 living adherent cells cultured on plastic dishes (2D) or >30 cells of H&E stained sections of paraformaldehyde fixed and paraffin embedded (FFPE) spheroids. Phase-contrast microscopy was performed using a Leica DMI6000B inverted microscope equipped with CTR6500 microscope drive control, DFC420C digital microscope camera with a 5 Megapixel CCD sensor and Leica Application Suite Version 3.8.0 software.