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. 2017 Nov 18;8(64):107477–107491. doi: 10.18632/oncotarget.22491

Figure 1. ILF3 interacts with HOXC8 in breast cancer cells.

Figure 1

(A) MDA-MB-231 cells were lentivirally transduced with the empty vector or vector encoding HOXC8-flag. Co-immunoprecipitations performed with the anti-flag M2 antibody were separated by SDS–PAGE, and the differential bands were analyzed by MS. (B) Co-immunoprecipitation (Co-IP) was performed using the M2 antibody in Hs578T or MDA-MB-231 cells that were lentivirally transduced with the empty vector or vector encoding HOXC8-flag. Immunoprecipitates were analyzed by Western blotting (WB). (C) Co-IP was performed using the ILF3 antibody in Hs578T or MDA-MB-231 cells that were lentivirally transduced with the empty vector or vector encoding HOXC8-flag. Immunoprecipitates were analyzed by WB. (D) Co-IP experiment was performed using anti-HOXC8 antibody in Hs578T or MDA-MB-231 cells, and precipitated proteins were analyzed by WB using anti-ILF3 or anti-HOXC8 antibodies as indicated. (E) Co-IP experiment was performed using anti-ILF3 antibody in Hs578T or MDA-MB-231 cells, and precipitated proteins were analyzed by WB using anti-HOXC8 or ILF3 antibodies as indicated. (F) Immunofluorescence staining for HOXC8-flag (green) and ILF3 (red) in Hs578T or MDA-MB-231 cells that were lentivirally transduced with the vector encoding HOXC8-flag; DAPI staining for the nucleus. Magnification, 200×; Scale bar, 100 μm.