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. 2017 Nov 18;8(64):107477–107491. doi: 10.18632/oncotarget.22491

Figure 2. ILF3 activates CDH11 expression in the Hs578T and MDA-MB-231 cell lines.

Figure 2

(A) Hs578T and MDA-MB-231 cells were transfected with siRNAs that specifically targeted ILF3 or with scrambled siRNAs. Cell lysates were immunoblotted for CDH11, ILF3 and β-actin, as indicated. (B) Hs578T and MDA-MB-231 cells were transfected with siRNAs that specifically targeted ILF3 or with scrambled siRNAs. Total RNA was subjected to qRT-PCR to examine the levels of CDH11 mRNA. β-actin mRNA was used as an internal control for standardization. (C and D) Hs578T or MDA-MB-231 cells were lentivirally transduced with NF90-, NF110- or ILF3-specific shRNA, and Western blotting was performed with antibodies for CDH11, ILF3 or β-actin, as indicated. (E and F) Hs578T or MDA-MB-231 cells were lentivirally transduced with NF90a, NF90b, NF110a or NF110b expression vectors, and Western blotting was performed with antibodies for CDH11, ILF3 or β-actin, as indicated.