Skip to main content
. 2017 Nov 18;8(64):107477–107491. doi: 10.18632/oncotarget.22491

Figure 4. Identification of the ILF3 binding sites in the CDH11 promoter by mutagenesis.

Figure 4

(A) A schematic diagram for the mutagenesis of four CTGTT (putative ILF3 binding) sites in the CDH11 promoter. (B) Luciferase analyses were performed with wild-type (WT) or mutant (Mut) CDH11 promoter luciferase reporter vectors in Hs578T or MDA-MB-231 cells. The luciferase activity was measured and normalized to the Renilla activity. Columns, mean; bars, SEM; **P < 0.01. (C and D) Hs578T or MDA-MB-231 cells were lentivirally transduced with NF90b, NF110b expression vectors or the empty vectors and then transfected with mutant (Mut) CDH11 promoter luciferase reporter vectors, as indicated. The luciferase activity was measured and normalized to the Renilla activity. Each sample was run in triplicate and in multiple experiments for mean ± SEM; *P < 0.05; **P < 0.01.