Figure 1. Large and small CRC cells can be prospectively sorted out by fluorescence-activated cell sorting (FACS).

(A) Cell size of the cultured cells in CRC cell lines (i.e., HCT116, SW480, LoVo and HT-29) was measured under microscope. (B) Schematic of large and small CRC cells sorting. (C) Post-sorting analysis of the sorted large and small CRC cells. (D) Sorted cells were plated on glass cover slides and cell morphology was observed under microscope. (E) Diameters of sorted large cells (i.e., L) and small cells (i.e., S) were measured under microscope. Data are presented from triple experiments; mean ± SD, ^*P< 0.05, ^**P< 0.01, ^***P< 0.001.