The SW620-shNCOA5 3# and SW620-shNTC; SW480-LV-NCOA5 and SW480-LV CRC cells s.c. injected into nude mice. The tumor volume (A) was measured after implantation of tumor cells.SW620-shNCOA5 3# compared with SW620-shNTC group: *, P<0.05 at week 2, and **, P<0.01 at week 3 and 4; SW480-LV-NCOA5 compared with SW480-LV group: *, P<0.05 at week 2, and **, P<0.01 at week 3 and 4, two-way repeated measures ANOVA, n=6 replicates per condition. The xenografted tumors were removed (B) 4 weeks after tumor cell's implantation and tumor weight (C) was then measured. SW620-shNCOA5 3# compared with SW620-shNTC group: **, P<0.01; SW480-LV-NCOA5 compared with SW480-LV group: **, P<0.01, Student t test, n=6 replicates per condition. Data shown were representative of three independent experiments.