Figure 1. α₂M^* signals through CS-GRP78 to regulate acetyl-CoA production and histone acetylation.

(A) Immunoblot analysis showing histone acetylation levels in 1-LN prostate cancer cell line after stimulated with α₂M^* at the indicated dose response for 30 min (left panel) and α₂M^* (100 pM) for the indicated time points. (B) Immunoblot analysis of the indicated cancer cell lines treated with C38 Mab (50 μg) for 6 h and then stimulated with α₂M^* (100 pM) for 30 min and acetate (5 mM) for 4 h alone or in combination. (C) Total cellular concentration of acetyl-CoA was measured in indicated cell lines treated with C38 Mab (50 μg) for 6 h and then stimulated with α₂M^* (100 pM) for 30 min and acetate (5mM) for 4 h alone or in combination. mean ± SEM of triplicates. (D) Immunoblot analysis of the indicated cancer cell lines treated with C38 Mab (50 μg) for 6 h and then stimulated with α₂M^* (100 pM) for 30 min and acetate (5mM) for 4 h alone or in combination. (E-F) Indicated cancer cell lines were treated with C38 Mab (50 μg) for 6 h and then stimulated with α₂M^* (100 pM) for 30 min and acetate (5 mM) for 4 h alone or in combination to quantify the transcript level of ACLY and ACSS1 genes. ^*, p values ≤ 0.05. Error bar represent S.D.