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. 2017 Nov 14;8(64):107977–107990. doi: 10.18632/oncotarget.22439

Figure 2. Effects of SHR8443 on PI3K/mTOR signaling in cancer cell lines with different genetic backgrounds.

Figure 2

(A) Breast cancer cell lines (MCF7, BT-474 and MDA-MB-468) and (B) KRAS or BRAF mutated cell lines (COLO205, SW620 and A549) were treated with increasing concentrations of SHR8443 or 100 nM BEZ235 for 3 h. (C) U-87MG cells were treated with 100 nM SHR8443 for the indicated time. (D) Reversibility of PI3K/mTOR signaling. MDA-MB-468 cells were treated with 100 nM SHR8443 for 2 h, with or without further culture in drug-free medium for the indicated time after washing with PBS. Whole-cell lysates were analyzed by Western blotting using the indicated antibodies.