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. 2017 Nov 18;8(64):108064–108078. doi: 10.18632/oncotarget.22514

Figure 3. Expression of EphA5, IGFBP5 and H2BK in cultured human non-stem GBM cell lines and primary cultures, and analysis of a Temozolomide (TMZ)-induced cellular dormant state in different GBM cultures.

Figure 3

(A) Cultured human glioma cell lines and primary cultures were analysed by qRT-PCR and Western Blot regarding the mRNA and protein expression of EphA5, IGFBP5 and H2BK (ΔCT 3.3 = 10-fold expression difference; black highlighted primary cultures correspond to solid GBM samples in Figure 1A). (B and C) GBM cells were stimulated with 500 μM TMZ or 0.2% DMSO (control) for 10 days, and the dormant state was analysed by monitoring dye retention at day 10 using combined transmitted-light and fluorescence microscopy (B), and determination of phospho-p38 / phospho-p42/44 ratios by Western Blot and subsequent densitometric analysis comparing DMSO and TMZ treated samples (C).