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. 2017 Nov 18;8(64):108064–108078. doi: 10.18632/oncotarget.22514

Figure 4. Induction and (co)-expression of dormancy- and stemness-associated genes during TMZ treatment in glioma cell lines.

Figure 4

(A and C) Non-stem GBM cell lines were stimulated with 500 μM TMZ or 0.2% DMSO (control) for 10 days and EphA5, IGFBP5 and H2BK (A) and OCT4, KLF4, MSI1 or SOX2 (C) expression was analysed in TMZ-stimulated compared to control samples using qRT-PCR (*p<0.05, **p<0.01, ***p<0.001). (B and D) Stimulated GBM cell lines were stained by immunocytochemistry to analyse the (co)-expression of IGFBP5, EphA5, H2BK themselves (B) and together with KLF4 and MSI1 (D). IgG antibodies served as secondary antibody controls.