Figure 6. Induction of dormancy- and stemness-associated genes during TMZ treatment in glioma cell lines, and determination of TMZ-induced and combined TMZ / AT101-induced cytotoxicity.

GBM cell lines were stimulated with 500 μM TMZ, 5 μM AT101 or 0.2% DMSO (control) for up to 10-12 days. 5 μM AT101 was added to TMZ stimulated cells at day 6, and stimulation was performed for additional 4-6 days. (A) Expression of H2BK and KLF4 was checked at days 3-6-8-10 using qRT-PCR, and (B) numbers of dead cells were determined at days 3-6-8-10-12 and documented as n-fold cytotoxic effects (^*p<0.05, ^**p<0.01, ^***p<0.001).