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. 2017 Dec 7;7(1):45–58. doi: 10.1002/sctm.17-0108

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© 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expression of neurological and mesenchymal cell markers in MiMPCs and MSCs during neurotrophic induction examined by immunofluorescence. MiMPCs and MSCs were cultured in NIM or growth medium (GM). Neurotrophically induced MiMPCs and MSCs were fixed and stained with antibodies against MSC markers (CD44, CD73, CD105) and Schwann cell markers (GFAP, p75/NGFR, and S100B). Neurotrophically induced and noninduced MiMPC and MSC cultures stained positive for all MSC and Schwann cell markers. Schwann cells were stained as a control, and were positive for all markers. All cells were counterstained with 4′,6‐diamidino‐2‐phenylindole dihydrochloride. All images were taken at ×10 magnification and are representative of all fields. Bar = 220 μm, n = 2. Abbreviations: GFAP, glial fibrillary acidic protein; GM, growth medium; MiMPCs, induced mesenchymal progenitor cells; MSCs, mesenchymal stem cells; NIM, neurotrophic induction medium.