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. 2017 Dec 27;7(1):146–154. doi: 10.1002/sctm.17-0003

Table 2.

Validation study showing consistency between cultures from the same or different primary culture

Primary culture Secondary culture Test
p63bright (%) K3+ (%) CFE (%) Viable cells (%) Cell yield
1 a 8.6 90.3 14.2 74.6 315,000
b 8.4 83.1 22.5 80.0 225,000
2 a 8.2 70.1 14.3 66.5 375,000
b 7.2 72.1 25.9 66.7 415,000
3 a 6.3 67.8 13.5 82.5 250,000
b 5.5 81.3 18.0 61.6 300,000
Mean 7.4 77.5 18.1 72.0 313,333
SD 1.3 8.8 5.1 8.3 72,296

Abbreviation: CFE, colony forming efficiency (measures the number of epithelial colonies that originate following culture).