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. 2017 Dec 20;7(1):68–77. doi: 10.1002/sctm.17-0149

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© 2017 The Authors stemcellstranslationalmedicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Integration of donor‐derived cells in the epithelial lung compartment. Immunohistological analysis was used to characterize the composition of the epithelial compartment within donor‐derived GFP+ green patches. Imaris software was used to determine fluorescence colocalization. GFP+ areas were designated as the areas of interest, and the colocalization channel identified pixels that included both red and green colors. ATI cells were stained with Aquaporin type 5 (A), ATII cells with surfactant protein C (B), club cells with CCSP (CC16) (C), and CFTR+ cells with anti‐CFTR (D). In each figure, the red channel represents the lineage marker, the green channel marks donor‐derived GFP+ cells, and the blue channel marks nuclear staining. Scale bar = 40 µm. (E): Summary of % ROI (average plus SD) colocalized 8 weeks after transplantation of 8E + 6 adult GFP+ lung cells (based on five different areas obtained from two transplanted mice). Abbreviations: GFP, green fluorescent protein; ROI, region of interest.