Skip to main content
. 2017 Dec 28;15(12):e2004310. doi: 10.1371/journal.pbio.2004310

Fig 7. Choline transporter-like 1 (CTL1) affects Arabidopsis PM-located auxin efflux transporter PIN-formed 1 (PIN1) and Arabidopsis PM-located auxin efflux transporter PIN-formed 3 (PIN3) recycling.

Fig 7

(A–D) PIN1-green fluorescent protein (GFP) localization in wild-type (WT) (A and C) and ctl1 roots (B and D) treated with brefeldin A (BFA) for 60 minutes before (A and B) or after (C and D) washout for 90 minutes. Insets at the lower right corner of panels A–D show enlarged views of PIN1-GFP labeled BFA bodies (from areas indicated by arrows). Scale bars are 20 μm. (I) Percentage of cells with PIN1-GFP labeled BFA bodies before and after BFA washout in the WT and the ctl1 mutant. Data are mean ± SD. Three independent experiments were performed. Five roots were measured for each experiment (Student t test, *P < 0.05). (E–H) PIN3-GFP localization in WT (E and G) and ctl1 (F and H) roots treated with BFA as shown for PIN1-GFP. Scale bars are 20 μm. (J) Percentage of cells with PIN3-GFP labeled BFA bodies before and after BFA washout in the WT and the ctl1 mutant. Data are mean ± SD. Three independent experiments were performed. Five roots were used for each experiment (Student t test, *P < 0.05). The raw data for panels I and J can be found in S1 Data.