Table 3. Performance of 4 sequence based diversity measures for non-B HIV-1 subtypes by env segments using AUC au ROC analysis.

Env segment	Diversity measure	AUC	95% CI	Optimal Cut-off	TPR (Recent)	TNR (Chronic)	N: recent/chronic)
GP120 C2_1	Percent complexity	0,805	[0,693–0,918]	0,0004	75%	72%	28/29
	Percent diversity	0,842	[0,743–0,941]	0,0231	100%	52%	28/29
	Shannon entropy index	0,844	[0,741–0,946]	0,0895	96%	66%	28/29
	Nb_haplotypes	0,826	[0,720–0,931]	8	96%	55%	28/29
GP120 C3_2	Percent complexity	0,759	[0,631–0,887]	0,0004	93%	55%	28/29
	Percent diversity	0,821	[0,705–0,938]	0,0216	93%	72%	28/29
	Shannon entropy index	0,795	[0,669–0,921]	0,0774	89%	72%	28/29
	Nb_haplotypes	0,849	[0,740–0,959]	7	93%	72%	28/29
GP120-C3	Percent complexity	0,718	[0,623–0,812]	0,0002	38%	62%	28/29
	Percent diversity	0,803	[0,720–0,886]	0,0222	88%	72%	28/29
	Shannon entropy index	0,767	[0,677–0,857]	0,0776	86%	64%	28/29
	Nb_haplotypes	0,812	[0,728–0,896]	7	95%	62%	28/29
GP120 C4	Percent complexity	0,698	|0,557–0,839]	0,0003	89%	55%	28/29
	Percent diversity	0,819	[0,702–0,936]	0,0235	96%	66%	28/29
	Shannon entropy index	0,752	[0,622–0,883]	0,1214	100%	48%	28/29
	Nb_haplotypes	0,756	[0,625–0,887]	7	96%	55%	28/29
GP120 V2	Percent complexity	0,762	[0,621–0,903]	0,0012	79%	67%	28/29
	Percent diversity	0,845	[0,732–0,959]	0,0174	92%	67%	28/29
	Shannon entropy index	0,766	[0,622–0,909]	0,1338	92%	62%	28/29
	Nb_haplotypes	0,761	[0,615–0,907]	8	92%	57%	28/29

TPR (true positive rate) = sensitivity: recent HIV-1+ specimens correctly classified. TNR (true negative rate) = specificity: chronic HIV-1+ specimens correctly classified. The optimal cutoff value indicates the highest accuracy (proportion of patients correctly classified) represented the highest TPR (recency) + TNR (chronic) or (Sensitivity+ Specificity).