Table 2. Sterol composition in N. fowleri treated with 0.1% DMSO or 0.2 μM posaconazole.
| Sterols | RTTca | % Composition | ||
|---|---|---|---|---|
| DMSO | Posaconazole | |||
| 4-Desmethylsterols (% of total) | 90.0 | 50.5 | ||
| 1 | Cholesterol | 1 | 25.6 | 26.1 |
| 2 | 7-Dehydrocholesterol | 1.044 | 12.5 | 3.9 |
| 3 | Lathosterol | 1.061 | 0.8 | |
| 4 | Zymosterolb | 1.068 | 0.4 | |
| 5 | Ergosterol | 1.114 | 26.7 | 14.2 |
| 6 | Ergost-5-enol | 1.136 | 1.4 | 0.7 |
| 7 | Ergosta-5,7-dienol | 1.202 | 21.3 | 5.7 |
| 8 | Ergost-7-enol | 1.22 | 1.2 | |
| 4-Monomethylsterols | 6.8 | 48.5 | ||
| 9 | 4α,14α-Dimethylcholest-8-enol | 1.082 | 1.1 | 15.5 |
| 10 | 4α-Methylergost-8-enol | 1.095 | 3.5 | 0.4 |
| 14 | 31-Norlanosterol | 1.143 | 2.2 | 29.7 |
| 11 | Δ7-31-norlanosterol | 1.238 | 2.9 | |
| 4,4-Dimethylsterols | 3.2 | 1.0 | ||
| 12 | 24-Dihydrolanosterol | 1.304 | 0.2 | 0.2 |
| 13 | 24-Dihydrocycloartenol | 1.324 | 1.1 | |
| 14 | Parkeol | 1.384 | 0.1 | |
| 15 | Cycloartenol | 1.406 | 2.0 | 0.7 |
| 14α-Methylsterols | 6.5 | 49.1 | ||
| Δ24(25)-sterols | 8.1 | |||
arelative retention time compared to cholesterol
bin bold are highlighted sterols constituting the mainstream of biosynthesis cascade in Fig 4