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(A) AMOTL2 peptide was injected to His6-LC8 immobilized on Tris-NTA sensor chip as described in Materials and Methods. Sensorgrams derived from five different analyte injections are indicated with colored lines. Black lines show the global fit of the experimental data to a 1: 1 Langmuir model. (B) Equilibrium analysis of the LC8 –AMOTL2 interaction at different LC8 immobilization levels.
