Fig 5. Action of Rubb₁₂-tri and Rubb₁₆-tnl on adult S. mansoni AChE and AP activity.

Five pairs of worms were cultured in Basch media for 24 h in the presence of a sub-lethal dose (5 μM) of Rubb₁₂-tri or Rubb₁₆-tnl and then incubated in AChE assay buffer or AP assay buffer. PBS extracts were then made from equal amounts of control and treated worms and 30 μg of each extract was used to determine somatic AChE activity by the Ellman method. (A) surface AChE (B) somatic AChE and (C) surface AP activity of control worms and worms treated with Rubb₁₂-tri or Rubb₁₆-tnl. For all assays, data are the average of triplicate biological and technical experiments ± SE. Differences were measured by ANOVA. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, **** P ≤ 0.0001.