Fig 6. Effect of Rubb₁₂-tri and Rubb₁₆-tnl on adult S. mansoni glucose uptake and storage ability.

Worms were cultured in Basch media for 24 h in the presence of a sub-lethal dose (5 μM) of Rubb₁₂-tri or Rubb₁₆-tnl. Worms were then incubated for 24 h in DMEM containing 1 mg/ml glucose. PBS extracts were then made from equal amounts of control and treated worms and 30 ug of each extract was used to determine the glycogen content of the worms using a modified glucose oxidase assay. (A) Amount of glucose in media collected from control and treated S. mansoni worms. (B) Levels of glycogen in extracts made from control and treated S. mansoni worms. For all assays, data are the average of triplicate biological and technical experiments ± SE. Differences were measured by ANOVA. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, **** P ≤ 0.0001. (C) Scanning electron micrographs of adult male S. mansoni worm tegument after incubation with 5 μM Rubb₁₂–tri; (I) intact tubercles of control worms; (II) withered tubercules of treated worms.