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. 2017 Oct 25;41(1):43–50. doi: 10.3892/ijmm.2017.3212

Figure 2.

Figure 2

miR-17 stimulates proliferation of VSMCs. (A) miR-17 mimic stimulated proliferation of VSMCs. VSMCs were transfected with miR-17 mimic, and cell proliferation was determined using MTT assay. (B) Regulation of cell cycle progression in VSMCs by miR-17. The proportions of cells in each cycle were examined using flow cytometry. (C) The expression levels of cell cycle-regulating factors PCNA and E2F1 were regulated by miR-17. The protein levels were determined using western blotting. Representative results were obtained from three repeats. *P<0.05 and **P<0.01 vs. miR-NC; #P<0.05 and ##P<0.01 vs. inhi-NC. miR, microRNA; VSMCs, vascular smooth muscle cells; inhi, inhibitor; NC, negative control; PCNA, proliferating cell nuclear antigen.