Table I.
Primary and secondary antibodies used in western blotting.
| Type of antibody | Antibody | Supplier details | Cat. no. |
|---|---|---|---|
| Primary | p65 | Cell Signaling Technology, Inc., | 8242 |
| RB | Danvers, MA, USA | Ab181616 | |
| PCNA | Abcam, Cambridge, MA, USA | Ab92552 | |
| E2F1 | Ab179445 | ||
| H3 | Cell Signaling Technology, Inc., | 4499 | |
| GAPDH | Danvers, MA, USA | Ab8245 | |
| Secondary | Goat anti-rabbit IgG H&L (HRP) | Abcam, Cambridge, MA, USA | Ab6721 |
| Rabbit anti-mouse IgG H&L (HRP) | Abcam, Cambridge, MA, USA | Ab6728 |
RB, retinoblastoma; PCNA, proliferating cell nuclear antigen; IgG, immunoglobulin G; HRP, horseradish peroxidase; GAPDH, glyceraldehyde 3-phosphate dehydrogenas.