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. 2017 Nov 7;41(1):293–301. doi: 10.3892/ijmm.2017.3240

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Basic characterization of J1 embryonic stem cells (ESCs) and parthenogenetic ESCs (pESCs) in vitro and in vivo. (A) Undifferentiated J1 ESCs and pESCs cultured on mouse embryonic fibroblasts formed colonies. (B) Immunofluorescence labeling of NANOG, octamer-binding transcription factor 4 (OCT4) and stage-specific embryonic antigen (SSEA)-1 in undifferentiated J1 ESC and pESC colonies. (C) Growth kinetics of J1 ESCs and pESCs (3 repeat̸timepoint; data represent means ± standard deviation). (D) Histological analysis differentiated elements of teratomas from J1 ESCs and pESCs 4 weeks after implantation in nude mice. Hematoxylin and eosin staining revealed that J1 ESCs as well as pESCs were able to form teratomas, containing ganglion (ectodermal), gland (endodermal) and cartilage (mesodermal) tissues. Scale bar, 100 μm. OD, optical density.