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. 2017 Nov 7;41(1):293–301. doi: 10.3892/ijmm.2017.3240

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Adipogenesis of J1 embryonic stem cells (ESCs) and parthenogenetic ESCs (pESCs). (A) Scheme used for ESC differentiation into adipocytes. (B) Adipogenic differentiation of J1 ESCs and pESCs was confirmed by upregulation of CCATT/enhancer-binding protein α (c/ebp α), fatty acid-binding protein 4 (fabp4) and peroxisome proliferator-activated receptor γ (ppar γ), and downregulation of oct4 by quantitative polymerase chain reaction at the ES and EB stages, at 5, 10, 15 and 20 days (3 repeat/timepoint; data represent means ± standard deviation). (C) Immunofluorescence labeling of adipogenic-specific factors c/ebp α and fabp4 during adipogenesis of J1 ESCs and pESCs in adipogenic induction medium at 5, 10, 15 and 20 days. (D) Image of Oil red O showing fat droplet formation at 5, 10, 15 and 20 days during adipogenesis of J1 ESCs and pESCs. (E) Statistical analysis of Oil red O-positive cells. Scale bar, 100 μm. ES, embryonic stem; EB, embryoid body.