Table I.
Genes | Primer sequences, sense/antisense (5′-3′) | Product size (bp) |
---|---|---|
fabp4 | ATGGCCAAGCCCAACATGAT | 135 |
CTTCCTGTCGTCTGCGGTGAT | ||
ppar γ | AAGGGTGCCAGTTTCGATCCGTAG | 154 |
ATCAGGGAGGCCAGCATCGTGTAG | ||
alp | ACAACCTGACTGACCCTTCG | 104 |
AATCCTGCCTCCTTCCACC | ||
ocn | TGGCCATCACCCTGTCTCCT | 229 |
GAGACCACTCCAGCACAACTCCT | ||
aggrecan | TGTAAGGACTGTCTATCTACACGCC | 147 |
TGGATGGTGATGTCGTCTTCAC | ||
Type II collagen | AAGACGGTGAGACGGGAGCC | 287 |
ACCATCAGTACCAGGAGTGCCAG | ||
oct4 | GATCACTCACATCGCCAATCA | 128 |
CTGTAGCCTCATACTCTTCTCGTT | ||
c/ebp α | AAGAGGACGAGGCGAAGCAGC | 132 |
GCGCGATCTGGAACTGCAAGT | ||
Alg3 | TACCTATCCCGCTCCTTTGAC | 171 |
CCCTGTTCTGTGCCACCTAC | ||
Alg12 | GGAGTCCTTGGGCTTGGTGTG | 191 |
CAGGCTGTGAGGGCTGGTAAGAC | ||
β-actin | CAGCCTTCCTTCTTGGGTAT | 100 |
TGGCATAGAGGTCTTTACGG |
RT-PCR, reverse transcription-polymerase chain reaction; qPCR, quantitative PCR; fabp4, fatty acid-binding protein 4; ppar γ, peroxisome proliferator-activated receptor γ; alp, alkaline phosphatase; ocn, osteocalcin; c/ebp α, CCATT̸enhancer-binding protein α.