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. 2017 Nov 7;41(1):293–301. doi: 10.3892/ijmm.2017.3240

Table I.

List of primers used for RT-PCR/qPCR.

Genes Primer sequences, sense/antisense (5′-3′) Product size (bp)
fabp4 ATGGCCAAGCCCAACATGAT 135
CTTCCTGTCGTCTGCGGTGAT
ppar γ AAGGGTGCCAGTTTCGATCCGTAG 154
ATCAGGGAGGCCAGCATCGTGTAG
alp ACAACCTGACTGACCCTTCG 104
AATCCTGCCTCCTTCCACC
ocn TGGCCATCACCCTGTCTCCT 229
GAGACCACTCCAGCACAACTCCT
aggrecan TGTAAGGACTGTCTATCTACACGCC 147
TGGATGGTGATGTCGTCTTCAC
Type II collagen AAGACGGTGAGACGGGAGCC 287
ACCATCAGTACCAGGAGTGCCAG
oct4 GATCACTCACATCGCCAATCA 128
CTGTAGCCTCATACTCTTCTCGTT
c/ebp α AAGAGGACGAGGCGAAGCAGC 132
GCGCGATCTGGAACTGCAAGT
Alg3 TACCTATCCCGCTCCTTTGAC 171
CCCTGTTCTGTGCCACCTAC
Alg12 GGAGTCCTTGGGCTTGGTGTG 191
CAGGCTGTGAGGGCTGGTAAGAC
β-actin CAGCCTTCCTTCTTGGGTAT 100
TGGCATAGAGGTCTTTACGG

RT-PCR, reverse transcription-polymerase chain reaction; qPCR, quantitative PCR; fabp4, fatty acid-binding protein 4; ppar γ, peroxisome proliferator-activated receptor γ; alp, alkaline phosphatase; ocn, osteocalcin; c/ebp α, CCATT̸enhancer-binding protein α.