Figure 1.

Metformin significantly inhibits cAMP-stimulated PEPCK expression. Primary mouse hepatocytes were incubated with 100 μM 8-br-cAMP in the presence or absence of 2 mM metformin for 8 h. PEPCK mRNA expression was detected by reverse transcription-quantitative polymerase chain reaction. Results are representative of three independent experiments. **P<0.01 vs. control group; ^##P<0.01 vs. cAMP group. cAMP, cyclic adenosine monophosphate; PEPCK, phosphoenolpyruvate carboxykinase; 8-br-cAMP, 8-bromoadenosine 3′,5′-cyclic monophosphate.