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. 2017 Nov 7;41(1):302–310. doi: 10.3892/ijmm.2017.3243

Figure 4.

Figure 4

Validation results of eight cAMP-upregulated and metformin-downregulated lncRNAs by RT-qPCR are consistent with those of the microarray. Primary mouse hepatocytes were incubated with 100 μM 8-br-cAMP in the presence or absence of 2 mM metformin for 8 h. RT-qPCR demonstrated that cAMP-stimulated (A) AK133602, (B) ENSMUST00000138573, (C) ENSMUST00000129953, (D) uc009njr.1, (E) AA591058, (F) NR_027710, (G) NR_030715 and (H) ENSMUST00000145208 were attenuated by metformin. All results were obtained from three independent experiments. *P<0.05 and **P<0.01 vs. control group; #P<0.05 and ##P<0.01 vs. cAMP group. cAMP, cyclic adenosine monophosphate; lncRNA, long non-coding RNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.