Figure 5.

Expression levels of PGC-1α and Gpr155 are increased by cAMP and are further decreased by metformin treatment. Primary mouse hepatocytes were incubated with 100 μM 8-br-cAMP in the presence or absence of 2 mM metformin for 8 h. (A) PGC-1α and (B) Gpr155 mRNA expression was detected by reverse transcription-quantitative polymerase chain reaction. The experiment was repeated three times with similar results. ^*P<0.05 vs. control group; ^#P<0.05 and ^##P<0.01 vs. cAMP group. PGC-1α, peroxisome proliferator-activated receptor-γ coactivator-1 1α, Gpr155, G protein-coupled receptor 155; cAMP, cyclic adenosine monophosphate.