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. 2017 Nov 1;41(1):137–146. doi: 10.3892/ijmm.2017.3229

Figure 3.

Figure 3

Effects of heat stress and cooling treatment on intestinal injury. Male Balb/c mice were used to establish heat-stressed mice, which were then divided into six groups: Sham-heated control mice (37°C), mice heated with Tc at 40°C or 42°C, and groups in which the animals were removed from the incubator and allowed to cool at an ambient temperature of 25±0.5°C for 6 h following core temperature reaching 40°C (40/6) or 42°C (42/6), or the sham control temperature (37/6). (A) Representative images of hematoxylin and eosin-stained ileal tissues (magnification, ×200). (B) Morphological changes in mice ileal tissues were assessed and graded in a blinded-manner by two certified veterinary pathologists using the Chiu intestinal injury score. Concentrations of DAO in (C) serum and (D) ileal tissues, and those of D-LAC in the (E) serum and (F) ileal tissues were analyzed using an ELISA. Data are presented as the mean ± standard deviation. One-way analysis of variance followed by the Newman-Keuls test was performed. *P<0.05 vs. 37°C group; #P<0.05 vs. 40°C group; △P<0.05 vs. 42°C group (n=6). DAO, diamine oxidase; D-Lac, D-lactic acid; HS, heat stress; CT, cooling treatment; ELISA, enzyme-linked immunosorbent assay.