Table II.
Oligonucleotide primers used in quantitative polymerase chain reaction.
| Gene | Primer sequence (5′-3′) |
|---|---|
| GAPDH | F: GACAGTCAGCCGCATCTTCT |
| R: TTAAAAGCAGCCCTGGTGAC | |
| RUNX-2 | F: TCAACGATCTGAGATTTGTGGG |
| R: GGGGAGGATTTGTGAAGACGG | |
| OCN | F: CCACCGAGACACCATGAGAG |
| R: TCAGCCAACTCGTCACAGTC | |
| ALP | F: ACCATTCCCACGTCTTCACATTTG |
| R: AGACATTCTCTCGTTCACCGCC | |
| PPARG2 | F: GCAAACCCCTATTCCATGCTG |
| R: CACGGAGCTGATCCCAAAGT | |
| LPL | F: CAAGAGTGAGTGAACAAC |
| R: AATTATGCTGAAGGACAAC | |
| SOX-9 | F: ACACACAGCTCACTCGACCTTG |
| R: AGGGAATTCTGGTTGGTCCTCT | |
| COL-2 | F: GGCAATAGCAGGTTCACGTACA |
| R: CGATAACAGTCTTGCCCCACTT |
ALP, alkaline phosphatase; COL-2, type II collagen; F, forward; LPL, lipoprotein lipase; OCN, osteocalcin; ; PPARG2, peroxisome proliferator-activated receptor γ, transcript variant 2; R, reverse; RUNX-2, runt-related transcription factor 2; SOX-9, sex-determining region Y-box 9.