Table II.

Primer sequences for the methylation analysis of mothers against decapentaplegic homolog 3 (Chr1:67356838-Chr1:67356942).

Method	Primer	Sequence (5′-3′)	Amplicon size (bp)
MSP	UF	TTTAGATATTGGTTAGTTGGTTGT	141
	UR	ACACATTAAAAAACTCCACAACTT
	MF	AGATATCGGTTAGTCGGTTGC	141
	MR	CATTAAAAAACTCCGCGACTT
Sequenom	F	AGGAAGAGAGGTTTTGTAATTTTTTATTTATTTGAGGG	352
MassARRAY	R	CAGTAATACGACTCACTATAGGGAGAAGGCTATTTTATTTCCAAAACAATAAACTCACA

UF, unmethylated forward; UR, unmethylated reverse; MF, methylated forward; MR, methylated reverse; F, forward; R, reverse.