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(A) Effect of AFE on the viability of 3T3-L1 preadipocytes determined by an XTT assay. (B) During adipogenesis of 3T3-L1, the reactive oxygen species levels were measured by fluorescence-assisted cell sorting. All values are expressed as the mean ± standard deviation. AFE, 70% ethanolic extract of Alnus firma; CON, control group; DMSO, dimethyl sulfoxide; DCFDA, 2′,7′-dichlorodihydrofluorescein diacetate; NAC, N-acetyl-L-cysteine.
