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. 2017 Nov 15;8(66):109877–109888. doi: 10.18632/oncotarget.22436

Figure 6. Exosome miRNA from cells to cells experiment.

Figure 6

(A) Schema of the labeling exosome miRNA-oligo experiment. (B) Validation of target cell delivery (Caki-1 to 769-P/RPTEC cells) using positive control using fluorescence microscope. Cell (769-P and RPTEC) imaging on fluorescence microscope using a standard RFP filter set to visualize the Texas-Red. signal conjugated to the XMIR-positive control oligo. (C) Schema: exosomes of Caki-1 transfected miR-224 inhibitor or miR-NC was added to 769-P cells and RPTEC cells. (D) Intracellular miR-224 expression in 769-P and RPTEC depending on extracellular miR-224 expression in Caki-1 cells