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. 2017 Nov 18;8(66):109894–109914. doi: 10.18632/oncotarget.22493

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Copyright: © 2017 Gangadaran et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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(A) A flow chart for the EV purification procedure based on ultracentrifugation. (B) Western blotting analysis of EVs. ALIX and CD63, EV marker proteins were detected by using anti-ALIX (97 kDa) and anti-CD63 (53 kDa) specific antibodies, respectively; GM-130 and Calnexin, EV negative marker proteins were detected by using anti-GM-130 (112 kDa) and anti-calnexin (90 kDa) specific antibodies, respectively; β-actin was used as a loading control.