Figure 3. Proliferation of UMUC-3^par and UMUC-3^res.

Temsirolimus-resistant cells were exposed to 1 μmol/ml temsirolimus three times a week. Tumor cells were further treated with VPA [1 mmol/ml] in the BrdU assay for 24 h or 48 h. Controls remained untreated. (A) BrdU incorporation [RFU] for each sample. (B) % difference of VPA treated cells to controls without VPA. Bars indicate standard deviation (SD). ^*indicates significant difference to control, ^#indicates significant difference to parental cells, p ≤ 0.05. n = 5.