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. 2017 Nov 29;8(66):110221–110233. doi: 10.18632/oncotarget.22753

Figure 6. Role of BST-2 in collective cancer cell invasion.

Figure 6

(A–C) Representative images of co-culture spheroids of shCTL (green) and shBST-2 (red), mono-culture spheroids of 4T1 shCTL and shBST-2, and mono-culture spheroids of 4T1 cells expressing BST-2 dimers (OE BST-2D) or a mutant BST-2 (OE BST-2DΔTy) embedded in Matrigel for the indicated times. Red boxes depict increasing invasive structures. White arrow heads indicate expanding spheroid boundaries while purple arrows show collective invasive structures. White dotted lines highlight the spheroid boundaries. Images are zoomed in rows 2 and 4. (D–F) Quantitation of mean fluorescence intensity (MFI) of co-cultured shCTL and shBST-2, mono-cultured shCTL and shBST-2, and mono-cultured OE BST-2D and OE BST-2DΔTy invasive strands respectively. Error bars represent SEM. Significance was taken at P < 0.05*. Experiments were repeated more than three times with similar results.