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. 2017 Nov 17;8(66):110234–110256. doi: 10.18632/oncotarget.22622

Figure 6. Effect of LINC00273 gene knockdown [using short interfering RNAs (siRNAs)] on B-16 melanoma cells.

Figure 6

(A) semiquantitative RT PCR expression of LINC00273 showing reduced expression in si LINC00273 treated B16 cells as compared to scramble control (sc+). (B) Densitometric analysis of LINC00273 expression measured by semiquantitative RT PCR showing efficiency of si RNA tranfection. (C) effect of siLINC00273 on MMP activity analyzed by gelatin zymography. MMP-2 and MMP-9 activities were evidenced at the corresponding molecular weight. (D-E) densitometric analysis of MMP-2 and MMP-9 activities. (F-G) Wound-healing assay to assess the effect of LINC00273 silencing on cell B-16 melanoma cell migration after 24hrs. (H) Effect of LINC00273 silencing on invasion ability was measured using transwell assays in B-16 melanoma cells. Microscopic observation (×10) of B-16 cells on the bottom of the Boyden's chamber at the end of the 24 h. (I) Optical Density (OD) and percentage of cells which have invaded to the lower chamber. (n=3 biological replicates, error bars denote s.e.m.). *p value<0.005 relative to Scramble Control.